General Question
How long can I leave this 1% agarose gel out in room temperature before electrophoresis?
My boss and I are doing an experiment with gel electrophoresis. The thing is, he’s horrible at time management and I don’t even know if he’ll be back before I leave work.
We already cast the agarose gel and it’s good to go. I was thinking of removing the combs and submerging the gel in TAE buffer at least while he’s away. But he’s experimenting with these different types of combs to place in the gel. I don’t want to mess it up with my unsteady hands. I’m also not sure if he has anything else in mind to do. Is it okay that the gel is lying there in the cast at room temperature? How long would it take to dry? Also as information – it’s not mixed with EtBr, we put Green View dye instead, if that makes any difference. We put a lot – like 20uL.
2 Answers
Answer this question
This question is in the General Section. Responses must be helpful and on-topic.