Would you expect the denatured DNA to regain its transforming capacity when cooled?

This sounds like a homework question.

Denatured proteins don’t, so I wouldn’t think DNA would.

Would you expect us to answer your homework question?

http://en.wikipedia.org/wiki/Denaturation_%28biochemistry%29

=)

@Makgowa, you’re new here, so let me just explain: flutherfolk do not welcome questions that essentially ask us to do your homework for you. There is also a strong tendency to be impatient with questions that can be answered easily by using a search tool such as Google.

We can help point the way to answers, do some coaching, and explain concepts that people don’t get, but generally speaking we think people should do their own studying and complete their own assignments. So why don’t you try to find out the answers for yourself, and come on back if you find them but have some trouble understanding what you learn?

And welcome to fluther.

no
would you expect a fried egg to become unfried when you cool it?

So, the answer to your question is a definitive YES. Sorry to all those that voted NO, but denatured DNA can efficiently transform bacterial cells. In fact, when cooled, it might partially renature, which would also enhance transformation.